strange that i cannot methylate the plasmid. is that enzyme out of work?
and the PCR of bisulfite come out, one can be use.
then the e.coli pcr , I FIND THE TRANS MIX IS VERY GOOD TO USE.
AND today is just not my day, l lost my card. have to pay 50yuan to buy a new one tomorrow.
thanks shidi buying me the supper. |